FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS983-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS983-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296907
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG785430.4540869647966541TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC41300.021.7109781
GCTTGAA321200.019.0685660
TGCTTGA320650.018.59912359
CTGCTTG324100.018.26048958
CTTGAAA375900.016.77795661
GCCGTCT404900.015.20410550
CCGTCTT416350.014.92887951
TGCCGTC422400.014.81456649
TCCGATC147450.014.6760795
TCTGCTT409250.014.64073657
TGAAAAA467000.014.53918863
GTGTGCT62500.014.3990722
ATGCCGT436500.014.37610848
TATGCCG437400.014.31452347
AAAAAGG254550.014.18941470
TGTGCTC82700.014.0611881
GAAAAAA488050.014.03397264
CTTCTGC442400.013.96291655
TTGAAAA470200.013.92664562
CCGATCT150900.013.8531226
Produced by FastQC (version 0.11.5)