FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12519010
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG248830.19876172317140092TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT127250.015.92466656
CATCTCG164250.015.34181446
TGCCGTC146700.015.22089555
TATGCCG159000.015.05584453
CCCCGGG12100.014.7515577
GTATGCC166850.014.74609352
ATGCCGT151550.014.47975454
CTGCTTG130750.014.45439564
TAGATCG76800.014.4077976
GCACACG297250.014.39955312
CCGTCTT148250.014.282757
CACACGT301150.014.22469413
AGTCACC286900.014.21166635
ACACGTC300650.014.20178814
CTCGTAT156950.014.1376649
CGTATGC170450.014.06505551
ACGTCTG304200.014.05906316
CAGTCAC290750.014.03551734
ATCTCGT174750.014.01943447
CACGTCT308400.013.91299215
Produced by FastQC (version 0.11.5)