FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9040878
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG225580.24951116473422164TruSeq Adapter, Index 10 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC32150.019.3927021
ACGTGTG21250.017.142481
CTGCTTG94950.016.4763558
GCTTGAA117550.016.13708560
TGCTTGA105050.016.05829659
AAAAAGG136900.015.49244870
GTGTGCT39700.015.4363952
TGCCGTC116650.015.18148449
TATGCCG131550.015.00502447
ATGCCGT120600.014.82934848
GCGTGAA32400.014.79871560
GTATGCC146050.014.54565146
GCCGTCT102650.014.52442950
CCGTCTT118850.014.34088151
TCCGATC89950.014.1705455
TGCGTGA29950.014.02273859
TGCCGGC34200.013.81516749
TATCTCG162900.013.81444840
CTGCGTG29400.013.80890158
CCGATCT90650.013.6746736
Produced by FastQC (version 0.11.5)