FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n01_DS795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n01_DS795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12671932
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG623540.4920638778680315TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC28950.018.511041
CTGCTTG266850.016.93230864
GCTTGAA283550.016.6383660
TGCTTGA279000.016.44582265
GCCGTCT305850.015.64246456
CCGTCTT313750.015.30431157
TGCCGTC321800.015.17166755
TATGCCG329200.015.01135753
ATGCCGT329150.014.9073154
TAGATCG94400.014.5407636
CTTGAAA334450.014.50398861
CTTCTGC327950.014.3752561
GTATGCC346550.014.35070752
TATCTCG348850.014.13564746
CGTATGC350950.014.06103551
TCTGCTT327300.014.01889563
GTCACGC366200.013.97250130
AAAAAGG208400.013.9223470
CGTCTTC347350.013.91457158
CTCGTAT352150.013.8938649
Produced by FastQC (version 0.11.5)