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        Note that additional data was saved in multiqc_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-06-02, 04:06 based on data in: /beegfs/mk5636/logs/html/H3WGNAFXY/merged


        General Statistics

        Showing 84/84 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H3WGNAFXY_n01_659T-1_sigW
        68.9%
        45%
        4.5
        H3WGNAFXY_n01_659T0_sigW
        57.6%
        44%
        1.7
        H3WGNAFXY_n01_79T-1_sigW
        69.5%
        45%
        4.1
        H3WGNAFXY_n01_79T0_1
        69.5%
        46%
        4.7
        H3WGNAFXY_n01_79T0_2
        66.4%
        43%
        2.9
        H3WGNAFXY_n01_79T0_3
        64.1%
        46%
        3.5
        H3WGNAFXY_n01_79T0_sigW
        64.9%
        45%
        4.1
        H3WGNAFXY_n01_79T1_1
        71.6%
        46%
        4.0
        H3WGNAFXY_n01_79T1_2
        69.8%
        44%
        3.3
        H3WGNAFXY_n01_79T1_3
        71.3%
        46%
        4.5
        H3WGNAFXY_n01_79T2_1
        69.7%
        46%
        4.1
        H3WGNAFXY_n01_79T2_2
        71.0%
        46%
        4.4
        H3WGNAFXY_n01_79T2_3
        66.0%
        46%
        3.7
        H3WGNAFXY_n01_79T3.5_2
        67.2%
        46%
        4.8
        H3WGNAFXY_n01_79T4.5_2
        60.3%
        46%
        3.9
        H3WGNAFXY_n01_OAT0_1
        60.4%
        47%
        1.6
        H3WGNAFXY_n01_OAT0_2
        60.2%
        44%
        2.2
        H3WGNAFXY_n01_OAT0_3
        69.7%
        45%
        5.7
        H3WGNAFXY_n01_OAT1_1
        69.3%
        45%
        3.6
        H3WGNAFXY_n01_OAT1_2
        61.7%
        43%
        1.7
        H3WGNAFXY_n01_OAT1_3
        67.0%
        45%
        3.9
        H3WGNAFXY_n01_OAT2_1
        56.4%
        46%
        1.3
        H3WGNAFXY_n01_OAT2_2
        70.8%
        45%
        3.0
        H3WGNAFXY_n01_OAT2_3
        66.0%
        45%
        3.8
        H3WGNAFXY_n01_VGT0_1
        70.8%
        47%
        3.2
        H3WGNAFXY_n01_VGT0_2
        66.1%
        44%
        4.0
        H3WGNAFXY_n01_VGT0_3
        68.3%
        45%
        4.9
        H3WGNAFXY_n01_VGT1_1
        71.7%
        47%
        4.0
        H3WGNAFXY_n01_VGT1_2
        65.2%
        43%
        2.2
        H3WGNAFXY_n01_VGT1_3
        69.1%
        46%
        3.8
        H3WGNAFXY_n01_VGT2_1
        71.0%
        46%
        4.4
        H3WGNAFXY_n01_VGT2_2
        73.4%
        44%
        5.2
        H3WGNAFXY_n01_VGT2_3
        67.5%
        43%
        3.5
        H3WGNAFXY_n01_WT-1_sigW
        90.2%
        44%
        0.7
        H3WGNAFXY_n01_WT0_sigW
        70.3%
        45%
        5.1
        H3WGNAFXY_n01_csfG3.5_2
        67.4%
        46%
        5.9
        H3WGNAFXY_n01_s8774.5_2
        53.4%
        46%
        2.5
        H3WGNAFXY_n01_sigE3.5_2
        74.0%
        46%
        6.0
        H3WGNAFXY_n01_sigE4.5_2
        41.6%
        46%
        0.6
        H3WGNAFXY_n01_sigF3.5_2
        71.6%
        46%
        4.8
        H3WGNAFXY_n01_sigG4.5_2
        51.6%
        45%
        0.4
        H3WGNAFXY_n01_undetermined
        66.6%
        44%
        7.5
        H3WGNAFXY_n02_659T-1_sigW
        66.3%
        45%
        4.5
        H3WGNAFXY_n02_659T0_sigW
        55.2%
        45%
        1.7
        H3WGNAFXY_n02_79T-1_sigW
        65.1%
        48%
        4.1
        H3WGNAFXY_n02_79T0_1
        64.6%
        50%
        4.7
        H3WGNAFXY_n02_79T0_2
        64.2%
        43%
        2.9
        H3WGNAFXY_n02_79T0_3
        60.9%
        48%
        3.5
        H3WGNAFXY_n02_79T0_sigW
        62.7%
        45%
        4.1
        H3WGNAFXY_n02_79T1_1
        68.4%
        49%
        4.0
        H3WGNAFXY_n02_79T1_2
        67.1%
        46%
        3.3
        H3WGNAFXY_n02_79T1_3
        68.0%
        49%
        4.5
        H3WGNAFXY_n02_79T2_1
        66.1%
        48%
        4.1
        H3WGNAFXY_n02_79T2_2
        68.5%
        46%
        4.4
        H3WGNAFXY_n02_79T2_3
        64.0%
        46%
        3.7
        H3WGNAFXY_n02_79T3.5_2
        64.4%
        47%
        4.8
        H3WGNAFXY_n02_79T4.5_2
        57.8%
        47%
        3.9
        H3WGNAFXY_n02_OAT0_1
        55.4%
        52%
        1.6
        H3WGNAFXY_n02_OAT0_2
        57.5%
        44%
        2.2
        H3WGNAFXY_n02_OAT0_3
        66.1%
        46%
        5.7
        H3WGNAFXY_n02_OAT1_1
        65.4%
        49%
        3.6
        H3WGNAFXY_n02_OAT1_2
        59.5%
        44%
        1.7
        H3WGNAFXY_n02_OAT1_3
        64.3%
        47%
        3.9
        H3WGNAFXY_n02_OAT2_1
        52.3%
        50%
        1.3
        H3WGNAFXY_n02_OAT2_2
        66.0%
        51%
        3.0
        H3WGNAFXY_n02_OAT2_3
        62.4%
        48%
        3.8
        H3WGNAFXY_n02_VGT0_1
        68.1%
        48%
        3.2
        H3WGNAFXY_n02_VGT0_2
        64.2%
        44%
        4.0
        H3WGNAFXY_n02_VGT0_3
        64.5%
        49%
        4.9
        H3WGNAFXY_n02_VGT1_1
        68.5%
        49%
        4.0
        H3WGNAFXY_n02_VGT1_2
        61.3%
        46%
        2.2
        H3WGNAFXY_n02_VGT1_3
        66.2%
        48%
        3.8
        H3WGNAFXY_n02_VGT2_1
        68.8%
        47%
        4.4
        H3WGNAFXY_n02_VGT2_2
        70.9%
        45%
        5.2
        H3WGNAFXY_n02_VGT2_3
        65.3%
        43%
        3.5
        H3WGNAFXY_n02_WT-1_sigW
        73.1%
        69%
        0.7
        H3WGNAFXY_n02_WT0_sigW
        65.4%
        48%
        5.1
        H3WGNAFXY_n02_csfG3.5_2
        64.9%
        47%
        5.9
        H3WGNAFXY_n02_s8774.5_2
        51.3%
        47%
        2.5
        H3WGNAFXY_n02_sigE3.5_2
        70.2%
        49%
        6.0
        H3WGNAFXY_n02_sigE4.5_2
        39.3%
        48%
        0.6
        H3WGNAFXY_n02_sigF3.5_2
        69.2%
        46%
        4.8
        H3WGNAFXY_n02_sigG4.5_2
        43.2%
        59%
        0.4
        H3WGNAFXY_n02_undetermined
        61.7%
        47%
        7.5

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 42/42 rows and 3/3 columns.
        Sample NameTotal Read CountPortion (%)Perfect (%)
        undetermined_library
        7,509,304
        4.9
        -1.0
        79T-1_sigW
        4,096,000
        2.7
        -1.0
        659T-1_sigW
        4,529,469
        2.9
        -1.0
        WT-1_sigW
        653,563
        0.4
        -1.0
        79T0_sigW
        4,103,057
        2.7
        -1.0
        659T0_sigW
        1,734,434
        1.1
        -1.0
        WT0_sigW
        5,121,067
        3.3
        -1.0
        79T3.5_2
        4,794,795
        3.1
        -1.0
        sigF3.5_2
        4,766,563
        3.1
        -1.0
        csfG3.5_2
        5,944,719
        3.9
        -1.0
        sigE3.5_2
        6,041,834
        3.9
        -1.0
        79T4.5_2
        3,854,251
        2.5
        -1.0
        sigE4.5_2
        571,536
        0.4
        -1.0
        s8774.5_2
        2,497,328
        1.6
        -1.0
        79T0_1
        4,666,807
        3.0
        -1.0
        OAT0_1
        1,562,640
        1.0
        -1.0
        VGT0_1
        3,190,830
        2.1
        -1.0
        79T1_1
        3,956,712
        2.6
        -1.0
        OAT1_1
        3,649,405
        2.4
        -1.0
        VGT1_1
        4,027,105
        2.6
        -1.0
        79T2_1
        4,126,017
        2.7
        -1.0
        OAT2_1
        1,256,615
        0.8
        -1.0
        VGT2_1
        4,432,278
        2.9
        -1.0
        79T0_2
        2,860,492
        1.9
        -1.0
        OAT0_2
        2,175,335
        1.4
        -1.0
        VGT0_2
        3,999,632
        2.6
        -1.0
        79T1_2
        3,301,804
        2.1
        -1.0
        OAT1_2
        1,723,773
        1.1
        -1.0
        VGT1_2
        2,220,293
        1.4
        -1.0
        79T2_2
        4,415,905
        2.9
        -1.0
        OAT2_2
        3,021,358
        2.0
        -1.0
        VGT2_2
        5,166,092
        3.4
        -1.0
        79T0_3
        3,477,288
        2.3
        -1.0
        OAT0_3
        5,712,958
        3.7
        -1.0
        VGT0_3
        4,928,912
        3.2
        -1.0
        79T1_3
        4,458,579
        2.9
        -1.0
        OAT1_3
        3,864,892
        2.5
        -1.0
        VGT1_3
        3,848,064
        2.5
        -1.0
        79T2_3
        3,717,377
        2.4
        -1.0
        OAT2_3
        3,780,873
        2.5
        -1.0
        VGT2_3
        3,472,199
        2.3
        -1.0
        sigG4.5_2
        398,164
        0.3
        -1.0

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        153,630,319
        153,630,319
        4.9
        1.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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