FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7509304
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1899602.5296618701280438No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTA147040.19581042397537776Illumina Single End PCR Primer 1 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78020.10389777800978625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC38300.040.9390528
TAGATCG37000.040.58398429
TGTAGAT48950.033.53974527
GTGTAGA49550.033.14047226
AGTGTAG50650.032.21442825
GGGGAAA166000.027.22418668
GAGTGTA61200.026.88692524
ACTCTAG7650.025.635235
GGGGGAA179350.025.1551867
CTCTAGT7750.024.4010496
GGAAAAA207300.024.300270
GGGAAAA195500.023.95855369
GGTGTAG19700.023.10071870
AGTGTAC10800.022.68090458
AGTGTGT17900.022.48179258
AGATCGG66950.022.37650530
TCTAGTT9300.021.4575827
TACGTGT11600.021.12403168
GTAGGGA82150.020.61431315
CTAGTTG9600.020.4193538
Produced by FastQC (version 0.11.5)