FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_sigF3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_sigF3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766563
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167260.35090273641615566No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG147800.31007667369549086No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC142270.29847502277846744No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC138220.2899783344938481No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA131700.27629971532947323No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG124220.26060706634948494No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA112000.23497014515490514No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC95690.20075261776672207No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC86960.18243753413098704No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT86360.18117876549622863No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG82510.17310166675652877No Hit
CTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATG57400.12042219939188888No Hit
CCGCCAGCTGCTACAGCGCGGCGCAATTCAAAAAGGCGAGGTTTCTTATA56190.11788368264512607No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT56090.11767388787266632No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT55530.1164990371468918No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA55190.11578573492052868No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG53930.113142320787536No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG53780.11282762862884639No Hit
TTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCT52930.11104437306293864No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT49960.10481346832088445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9400.031.6522776
TTAGAGT13150.029.53568612
CCTTAGA13400.028.98586310
CTTAGAG13600.028.81659311
TCTAGTT10450.028.8059647
GGCGTGA27000.027.6209811
ACCTTAG13950.027.5965569
ACTCTAG10950.026.8527135
TGTACTC11650.025.5431982
ACACGAG14250.025.29534128
CTAGTTG12050.024.6869988
TAGTTGT12800.023.7873299
CTAGATT13000.023.42309257
CACCTTA17350.022.5920128
GAGTACA17700.021.94294715
TGTTACC13050.021.71799713
GTGATAG36300.021.6005293
GAGTAAC15250.021.3456250
GGGGGAA33600.021.2549267
ACCTCTA13700.021.1986117
Produced by FastQC (version 0.11.5)