FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_sigE3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_sigE3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041834
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1719382.8457915262153843No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG185480.3069928766662573No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC116650.19307051468146924No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA110120.18226253816308094No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG108640.17981295083578924No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA100930.1670519249618576No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC96270.1593390351340338No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC85200.14101678397652104No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT73300.12132077776383793No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG73230.12120491890376332No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT63520.10513363988484291No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT63470.10505088355621818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9050.035.9675946
TCTAGTT10250.032.438357
ACTCTAG11550.028.4856725
GGGGGAA217700.028.10789367
GGGGAAA212500.027.82400368
GGAAAAA222900.027.13897170
TACAAGG53750.025.4565648
TTAGAGT15550.024.97720112
GGCGTGA33400.024.1090771
CTAGTTG13850.023.7508728
CCTTAGA16550.023.6798210
ACCTTAG17100.023.5344059
GTGATAG37700.023.303064
TGTACTC13850.023.2526572
TAGTTGT14500.023.1688619
GGGAAAA258100.022.97656469
CTTAGAG17250.022.92174711
TACCTCT14500.022.44223416
ACCTCTA15300.022.18357317
GGGGGGA298300.022.05070166
Produced by FastQC (version 0.11.5)