FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_s8774.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_s8774.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497328
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142330.5699291402651153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA22200.020.18720267
ACTCTAG3000.019.8362625
GGGGGGA27800.017.5060966
TCTAGTT4200.017.5015347
CTAGTTG3850.017.2697438
GGGGAAA27550.016.64826268
GTCGACT3005.511538E-1016.341
CTCTAGT4050.015.5578526
TAGGTCC1708.179377E-414.41187347
CCTACTA2759.150772E-714.00290934
TAGTACG1800.00126125813.60930424
GGGAAAA34750.013.60212769
ACCTACT3405.0462404E-813.38513433
GTACCAC1850.001547386413.2451985
TGGTAGT1850.001553233813.23856915
ACCTCTA4601.0186341E-1012.93021717
GGCTAGA1900.001893357612.89483351
GACACCT6550.012.82657830
GGGGGTA3305.0836206E-712.73170366
CCCTAAG3952.6790076E-812.40217522
Produced by FastQC (version 0.11.5)