FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_csfG3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_csfG3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944719
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG684081.1507356361166945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA113150.026.94666567
GGGGAAA122200.024.69320768
GGGGGGA145550.021.33304466
ACTCTAG7100.021.1988035
CTCTAGT7650.021.0471846
CGGTGGT6650.020.00119252
GGGAAAA155000.019.8077469
GGAAAAA176300.018.26848470
CTAGTTG8450.018.223648
TTAGAGT11550.018.17816212
TCTAGTT9050.018.1780327
CCTTAGA12450.017.42709210
TCGGTGG7650.016.92854551
ACCTTAG12950.016.4853889
CTTAGAG13550.015.75368611
GGTGGTC9050.015.47053653
GTGATAG17950.015.2099493
GTAGATC10400.014.47165244
GTAGGGA11100.014.18693219
TAGTTGT12100.014.1726099
Produced by FastQC (version 0.11.5)