FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_WT0_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_WT0_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121067
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG968201.8906216224079866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG107280.20948759311291962No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG71050.13874061792200726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG70470.13760804144917457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG68140.13305820837727764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT7400.037.3632437
TCGGTGG6750.036.81572751
GGAAAAA312500.033.38302270
GGGAAAA322450.030.82211769
GGGGAAA321050.030.41013368
ACTCTAG6400.030.081815
GATAGGG9600.029.89453136
GGGGGAA324150.029.84932167
GGATAGG9800.028.21746835
TACTCTA7950.028.1795524
TAGGATA10500.026.33630433
GTGGTCG10150.025.86321654
CTCTAGT7950.025.0971686
GTAGGAT12900.024.69270532
GGTGGTC10350.024.34890653
GGTCGCC11050.024.07299656
GGGGGGA419000.023.35125566
CGGTGGT11650.021.63207252
GTAGGGA13000.021.53519
TGGTCGC13050.021.18826555
Produced by FastQC (version 0.11.5)