FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472199
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111930.3223605559473982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT3550.019.7262971
TAGGGAG5150.019.70990632
GGGAGTT6000.016.9176734
TGTGCTA4600.015.97483518
ATAGGGA6800.015.95659831
GGGGGGA21250.015.48639366
GGGTAAT8000.015.3187031
CTAACGT11700.014.9633241
TAGTCGA5100.014.4099229
GGGGAAA20300.013.62424568
AGGATCC1800.001258921113.6128983
CTATAAC6450.013.563374511
GCTAGAT4751.2732926E-1113.2685311
CTAGGGC9800.013.214498564
CTAGGTA5300.013.2115652
GGGGGAA21750.013.19884767
TGTGGTA3507.4149284E-812.99758724
CTCAACT9750.012.9283121
ATTAAGT9550.012.8287256
AATAGGG8500.012.76527930
Produced by FastQC (version 0.11.5)