FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5166092
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108100.20924908034932402No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG60900.11788407949374499No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA59640.11544509853870198No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA52790.102185559219619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAG10700.029.4333720
CAACTAG11100.028.6879618
CTAGAGT11000.028.63064221
CAATAGA11500.028.3033539
CCAATAG11650.027.6385138
ATTAGAG13500.026.4458167
AGAGGTA14200.023.90344210
GGTAACA14100.023.32754113
GTACAAT14000.023.24760626
GTGATAG19400.023.0955473
ACTCTAG7300.022.0574345
GAGGTAA15550.021.8275911
AACTAGA15050.020.9260519
CTAACCT7300.020.6163142
TAGAGGT17150.020.610129
CGGGGAG9000.020.229541
CGTGATA23550.019.9191912
ACAACTA17450.019.85199717
GGGGAAA48050.019.23431868
GGGGGAA50500.019.13285467
Produced by FastQC (version 0.11.5)