FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432278
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496411.1199884122791937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT10850.041.2940066
TCTAGTT11500.040.1770447
ACTCTAG11400.039.9167485
CTAGTTG13550.035.1259468
GTACTCT13450.034.094193
TACTCTA16400.030.094834
GGGGGAA74600.026.23125367
GGGGAAA74250.025.8362968
TGTTACC17500.024.99292613
TGTACTC18600.024.8442942
ACCTCTA18100.024.16470317
TACCTCT18100.023.97138616
TAGTTGT21300.023.3312059
GGGGGGA92150.022.45138466
GGGAAAA87900.022.26350269
TTACCTC20000.021.69410315
TGGACAC21300.020.53710628
GACACCT21300.020.37717630
GGAAAAA101050.020.26702970
TTGTTAC22600.019.81784612
Produced by FastQC (version 0.11.5)