FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3848064
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG843702.192531101353824No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC86090.2237228902637794No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66020.17156679306789074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT15400.046.3511921
CCAATAG15900.042.26214238
ACTAGAG17100.041.74317620
AACTAGA17050.041.6603719
AGAGGTA18100.041.56072210
CAATAGA16400.040.97366339
CAACTAG17600.040.95491418
AGAGTAC18950.037.30159823
TAGAGTA19300.036.9877722
TCGCTTA15000.035.9372352
GGTAACA20800.035.82753413
TAGAGGT21250.035.569699
GAGGTAA21250.035.3999611
AGGTAAC21400.035.31440412
GTACAAT20450.035.2513826
ATTAGAG21950.034.4443057
ACAACTA22600.032.51001417
ATAGAAT21400.031.89183641
CCTACTA7850.031.66112934
TAGAATC21200.031.5323442
Produced by FastQC (version 0.11.5)