FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220293
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314211.4151735829460346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG37670.1696622923190768No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG24510.11039083580410333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG24330.1095801319915885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG23870.10750833335960615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG4500.043.5523938
GTGGTCG4750.040.52884754
TCGGTGG4400.039.7734251
CGGTGGT4750.039.05507352
GGTCGCC5200.037.69296356
CCAATAG3250.034.45903438
GTAGGAT6300.033.33766632
GGTGGTC6000.033.25207553
GGAAAAA111050.033.04190470
TAGATCT5550.032.794145
GGGGAAA105700.032.22854268
GGGAAAA109850.031.84108269
ATAGGGT6150.031.29853837
GGGGGAA107750.031.29045167
GAGTGTA6250.030.79429228
GATAGGG6250.030.23643936
CTAGAGT3950.030.1196421
TGGTCGC6400.030.07864655
AGATCTC5850.029.91572246
AGGGTGT6350.029.76161639
Produced by FastQC (version 0.11.5)