FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027105
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG779301.9351370277159399No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG55960.13895838325546514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT8850.035.2012636
GTACTCT9350.034.0671583
TACTCTA10450.032.8258444
TCTAGTT10100.032.2301457
ACTCTAG9900.031.1142235
CTAGTTG10250.029.7047568
GGGGAAA112050.028.83627368
GGGAAAA113600.028.7201669
GGGGGAA113650.028.55351367
GGAAAAA122550.027.45107570
TACCTCT11350.026.51292616
TGTTACC11950.024.88861713
ACCTCTA12300.024.46518117
TAGTTGT14650.021.9776279
GGGGGGA156400.021.71122266
TGTACTC15050.021.3988192
TGGACAC14150.020.527528
GTTACCT15450.019.7033714
TTACCTC16400.019.41544215
GACACCT15500.018.96846230
Produced by FastQC (version 0.11.5)