FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90220.22557075250923087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT3400.022.6532421
AGTCTAG2453.092282E-1119.99620614
GGCCTAC1257.816142E-519.6053521
CTAGGTA4950.015.5580522
TGGCAAC13700.014.0496599
GTCTAGG3556.009941E-913.80019815
TCTAGGC4152.728484E-1013.49141716
CTAGGCT4152.728484E-1013.49141717
TGGGAGT5500.013.365782
TATCGAC12950.013.2438775
TTACTCG14100.013.15600142
AGTATAC11300.013.00947231
CTACTAG4651.200533E-1012.79647134
GAACTAC8100.012.5316213
GGGGGGA19750.012.40782966
GTAGTAT7600.011.97141413
GGCAACT15550.011.92699810
TACTCGT16050.011.77567743
TAGCTCG5401.1823431E-1011.6663539
GAGTATA12100.011.57080230
Produced by FastQC (version 0.11.5)