FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_VGT0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_VGT0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190830
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG356821.118267033969218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA4250.030.472194
ACTCTAG4400.028.6378735
GTACTCT4450.028.3160953
GGGGGAA59550.028.1003667
GGGGAAA61500.027.43706968
GGGAAAA69450.024.80117469
CTAGTTG5150.024.4627068
TCTAGTT5800.023.5349837
CTCTAGT5550.022.703546
GGGGGGA79200.022.01287566
GGAAAAA82950.021.9043570
TACCTCT5700.020.87240416
TACCCCC1803.6645451E-617.49937
ACCTCTA7150.017.12893717
TGGACAC7250.016.41182728
TAGTCTA2151.0982749E-616.27992648
ACTAGTG1300.002291670516.1541955
TGTTACC7700.015.90568913
CTAATGC8500.014.8212921
TGGTACC4550.014.61890366
Produced by FastQC (version 0.11.5)