FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780873
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1534224.057845899611015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50100.1325090792523314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG39690.10497575559930206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA193400.032.4188270
TCGGTGG6800.031.39899851
GGGAAAA192750.031.23864269
GGGGAAA192750.030.87499268
GGGGGAA202250.029.64975767
AGTGTAC7800.029.61721629
GGTGGTC7400.028.85351653
GGTCGCC8000.027.5631156
CGGTGGT8100.026.3652
TAGATCT7600.024.40875645
GTGGTCG8900.023.59758854
GGGGGGA265450.023.54007566
GAGTGTA10200.023.33045628
GTAGATC8400.022.91747744
GTCCTGG8900.022.02033636
TGGTCGC10650.022.01955855
GTAGGGA11450.021.3938919
AGAGTGT11050.021.21910327
GTCGTGT11950.021.08359114
TCCTGGT9800.021.07050137
Produced by FastQC (version 0.11.5)