FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615711.5930846191821144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68270.17664141714697332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA83050.027.52510667
GGGGAAA83200.027.3913368
GGGAAAA97250.023.54201569
GGGGGGA104150.023.15876866
GGAAAAA113500.021.03507270
GGGGTAA8600.015.87528867
GGGGGTA9550.015.3957766
GATTCGA13150.014.1078434
GGGGTCT3507.372182E-813.0034591
TAGACTC8500.012.76479853
CGGCCTA6500.012.38470548
ACTGCGC8350.012.15357511
ATTCGAC16000.011.8136555
GGTGGTC5156.6393113E-1011.55348153
AACTAAC24200.011.28107363
GGATTCG18400.011.2239253
CCTAACG6251.0913936E-1111.20008151
AGACTAG5005.145921E-911.19993659
AGACTCC9400.011.17029254
ATCCGAG9800.011.07365868
Produced by FastQC (version 0.11.5)