FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723773
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48330.2803733438219534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC3850.022.7236311
CCTAGGT1553.499008E-822.5881611
GGTACCT800.002669268521.87275921
CGTAGTA2108.367351E-1121.6631912
CTAACGT4250.019.7712881
GGGGGGA9750.017.59253766
GCTAGAT1000.00967372317.5058251
AGGTACC1000.009697990517.49820920
AACTATA5650.016.7234679
GGGTAAT2753.0686351E-916.5509621
ACCCGCG5550.015.76643944
GGGGGAA9850.015.63767
CTAGGTA2251.760669E-615.5584772
GTGCAAC1350.002947705615.5553176
GTAGTAT3151.1114025E-915.553061513
GTCTAAC2755.491529E-815.2778111
CGTAGGC1859.039696E-515.13490335
ACTATAA6400.014.76325610
TCTAACG3101.4986654E-814.6801762
CCTACTA1708.1807753E-414.41112531
Produced by FastQC (version 0.11.5)