FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649405
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1390573.8104019696361466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG121190.33208153109890515No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA46020.12610274825622259No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38200.10467459763988925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG12550.049.0888945
CTCTAGT13650.045.6458856
GTACTCT13800.044.3887863
TGTACTC14550.042.1064762
TCTAGTT16600.037.5325747
CTAGTTG18100.033.6412358
TTGTACT18400.033.300261
TACTCTA19400.033.1994134
GGAAAAA203500.032.5656770
TAGTTGT19450.031.4861589
GGGAAAA202150.031.20720569
GGGGAAA205550.030.17964468
ACCTCTA19700.029.66345417
TGTTACC19950.029.4671313
GGGGGAA209800.029.35136267
TACCTCT19850.029.26301416
TTACCTC21550.027.27931615
GTTACCT21750.026.86758814
GTTGTTA26050.023.50724811
CACCTAC8850.023.33485832
Produced by FastQC (version 0.11.5)