FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5712958
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149400.2615107620255566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96550.1690017675606927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA122750.027.15066767
GGGGAAA123650.027.00943468
GGGAAAA139900.024.42353669
GGGGGGA151100.022.4967566
GGAAAAA176500.020.21184370
AGTGTAG4950.017.67653741
AGTCTAG2056.6968096E-717.06951314
TAGTGTA5850.016.75191940
GGGACTA2201.3944646E-615.9120872
AACTATA10650.015.1157199
GGTGGTC6750.014.00055453
CTATAAC10800.013.93244211
GTGGTCG6600.013.78878554
GGGTTTT57200.013.09320336
GGGGTTT53350.012.85910335
GTGGGTT17450.012.83729634
TTGTGGG25150.012.80346345
AGATTAG7200.012.63772836
GGGGTAA15850.012.58956167
ACTATAA12700.012.39924210
Produced by FastQC (version 0.11.5)