FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175335
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97260.4471035495682274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT2155.456968E-1222.7961481
GTCGACT800.00266420421.8802321
ACCCACG1454.6202877E-721.72034512
GGGTAAT4100.019.6388421
GGGGGGA15300.018.98972366
CTAGGTA2607.2759576E-1118.849362
GGGATCC3750.016.80247166
TAGGAGT2108.6067485E-716.66375514
TGGGAGT2151.0965341E-616.281842
CCACGTA1300.002289079116.1565932
GGGGGAA15600.016.15622367
AACCCAC2302.2211261E-615.21543211
ACACGAC8200.014.94019253
GGGGATC4301.8189894E-1214.65331865
TCAGTCG8750.014.39814710
GGGGAAA16300.014.17386568
CGTAGTA2001.7574686E-413.99755412
AGGTAAG3801.0495569E-913.8159164
GGATCCG4455.820766E-1113.37275467
GTCTAAC2102.6482836E-413.3365221
Produced by FastQC (version 0.11.5)