FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_OAT0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_OAT0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562640
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1027096.572787078277786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG88450.5660292837761737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG23630.1512184508268059No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG19750.1263886755746685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG16620.10635847028106281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA3350.037.6144184
GGAAAAA128150.036.47058570
GTACTCT3600.035.0023083
TCTAGTT3800.034.0800977
GGGAAAA143950.031.56770969
CTCTAGT3900.031.4123276
GGGGAAA148050.029.65303668
GGGGGAA151900.028.8553767
ACTCTAG4250.028.8254285
TGTACTC4800.025.5265982
GGTGGTC1951.8189894E-1225.12905753
GAAAAAA80650.024.26540270
TACCTCT5150.021.74274316
GGGGGGA206500.021.73441766
CCGTGTA3100.021.45165340
TAGTTGT5900.021.3525549
CTAGTTG6000.020.9966778
GTGTAGA2850.020.87723242
CTAGTGG1601.186585E-619.68816656
CGGGAGT1257.8150806E-519.6044272
Produced by FastQC (version 0.11.5)