FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T4.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T4.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3854251
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG508921.3204121890349123No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG39190.10167993729520988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG5500.035.0020945
TCTAGTT6950.030.216467
CTCTAGT6650.028.9491026
CTAGTTG6950.028.7019128
TAGTTGT7900.027.4653669
GTACCAC2150.026.048075
GGGGGAA74200.024.62731267
CCTTAGA6450.024.41436410
TCGGTGG4650.023.33412651
GGGGAAA77250.023.24712668
TTAGAGT6700.022.4579112
ACCTTAG7350.022.3785179
ACCTCTA9450.022.21720917
GTGTAGA6500.021.00016642
ACCTACT5950.020.58920133
CGGTGGT5500.020.36538552
CTTAGAG7800.020.1885411
GGGAAAA94450.019.31092569
TGTACTC10250.019.125582
GGGGGGA102000.019.01340966
Produced by FastQC (version 0.11.5)