FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4794795
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG801131.6708326424800226No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG181790.3791402969261459No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA122030.2545051456840178No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG106860.22286667104641594No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC104210.2173398445606121No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA91800.19145761184784751No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC77330.16127905364045805No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC67720.14123648664854285No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC62300.12993256228889868No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT57470.11985913892043351No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG51650.10772097660066801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT13150.040.7246
ACTCTAG13600.039.891245
TCTAGTT14400.037.674677
TGTACTC15450.036.479982
CTAGTTG17550.030.7092678
TAGTTGT19300.028.4687379
GGGGGAA135600.027.54506767
GGGGAAA140200.026.64130868
GGAAAAA164850.024.48460270
TGTTACC21450.024.47136113
TTGTACT23350.024.4406261
GGCGTGA26350.023.7839531
ACCTCTA21850.023.22259317
TACCTCT22400.022.65239716
TTACCTC23100.022.42042715
GTTACCT23600.021.79713814
GTTGTTA25350.021.67284211
GTGATAG30800.021.4783233
GGGGGGA178850.021.1580666
TTGTTAC24800.020.88354312
Produced by FastQC (version 0.11.5)