FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3717377
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG214160.5761051408022377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54370.1462590423301161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGGG952.781927E-422.10655459
GGGGGAA35850.021.87400267
GGGGAAA39150.020.03021868
GGGGGGA43250.019.34555266
GGGAAAA44950.017.60191269
TTAGTAT2701.1691125E-512.96023311
GGAAAAA68250.012.51607770
TAGGGTT7600.012.43242822
TAGATCT4852.4374458E-1012.26859345
TCGGTGG4306.6411303E-912.20985251
GATTAGT2601.1790135E-412.1139749
AGTGTAC4701.9463187E-911.91526929
CTAATTA2651.405997E-411.8873273
GGTCGCC6209.094947E-1211.29067956
TACCCTA2200.00568196711.13401816
GTCCTGG5158.174538E-910.87310536
TATACTA2600.001599373810.76725218
GAGTGTA5251.1044904E-810.66542528
CTCGACG5956.4392225E-1010.58600617
GGTGGTC5006.1474566E-810.50075553
Produced by FastQC (version 0.11.5)