FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4415905
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG98500.22305733479320772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82140.18600943634430542No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC73750.16700993341115808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAG15300.046.2017920
CTAGAGT15250.045.89432521
CAACTAG15100.044.72847418
AACTAGA16050.042.08099719
AGGTAAC17050.042.0739812
CAATAGA16800.040.20784439
GGTAACA17900.039.4900213
CCAATAG17350.038.3276338
TCGCTTA15550.037.8143352
GTACAAT19800.035.88096626
ATTAGAG20450.035.5993967
AGAGGTA21150.034.41454310
TAGAGGT21600.033.699489
ACAACTA20850.033.39919717
GAGTACA22050.031.58477424
TAGAATC21100.031.3510442
GAGGTAA23600.031.13804811
ATAGAAT22150.030.49693341
ACATCGC22900.029.1931149
CACATCG22950.028.97699548
Produced by FastQC (version 0.11.5)