FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126017
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1269603.077059546773559No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG99470.24107995677186983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9500.039.0549246
TCTAGTT10850.034.8407557
ACTCTAG11500.031.9583995
CTAGTTG11250.031.7305818
GGAAAAA190950.031.00210670
GGGGAAA190600.029.49674468
GGGAAAA196200.029.15548569
GGGGGAA193800.028.99163467
TGTACTC13750.027.749442
TGTTACC14750.023.48578813
ACCTCTA14950.022.93781917
TACTCTA18250.022.823264
GTACTCT17850.022.7464333
TACCTCT15800.022.14675516
GGGGGGA267350.021.69670766
TAGTTGT17850.021.5667579
TTGTACT18350.020.797211
GTTACCT16750.020.47260914
TTACCTC18200.019.61059415
GGGGTAA18100.018.95391767
Produced by FastQC (version 0.11.5)