FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458579
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1345963.018809356075108No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC57150.1281798528185774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT10400.039.70271321
GAGTGTA9400.033.506228
AGTGTAC9250.032.9223129
ACTAGAG12400.032.73465720
TCGGTGG8950.032.45926351
CCAATAG11900.032.3519238
CAATAGA12400.031.89415439
TAGATCT9050.031.71277445
GGTCGCC10300.030.92308856
GGAAAAA186600.030.676970
CGGTGGT9950.030.60513952
GGGGAAA178050.030.45689268
GGGAAAA180350.030.41993369
GGTGGTC10450.030.14529453
AACTAGA13500.030.06739219
TAGAGTA13400.030.03232222
GTGTAGA10900.029.8621142
GGGGGAA181900.029.7163667
AGAGGTA14200.029.56956710
GTAGGGA10800.029.48418819
Produced by FastQC (version 0.11.5)