FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301804
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG737152.2325674086045084No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG139420.422254016289277No Hit
AAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAA75030.2272394121516601No Hit
GGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTG71070.21524596856748612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG61940.18759441808175167No Hit
AAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAGAAAACAA51600.1562782042786307No Hit
GGCAATTTCAAAATCAGGGATAGGACCGTCCACTAGACAAGCGGCTCCGA47180.14289158290437592No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC46870.14195270221975623No Hit
TAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGTCCACTA46680.14137725921950545No Hit
GAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGCTCGATCG42500.12871751321398847No Hit
ACCGAATAGACCTGTTGCACCGGCAATTTCAAAATCAGGGATAGGACCGT34200.10357974004513897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA107900.029.88272968
GGGGGAA110850.028.676967
GGGAAAA118100.027.68761869
GGAAAAA122600.027.64225870
CCAATAG5700.027.0172738
CTAGAGT6150.025.03394921
CCGTGTA7250.024.62081140
GGTCGCC5500.023.54628656
GTAGGGA8850.023.3270919
GTCGTGT8950.023.06610114
GGTGGTC5400.022.68701753
ACTAGAG7000.022.4939820
TGTAGGG9200.022.43964618
CGTGATA9700.022.376672
AGAGCCG9150.022.18388736
CTAGGTC800.002670699321.8714648
AGGGAGG48350.021.56577114
AGAGTGT8950.021.50620727
GGGGGGA152550.021.43434166
ACGGTCC16350.021.41267269
Produced by FastQC (version 0.11.5)