FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956712
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG876392.214945136264656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG78350.1980179502576887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG8750.040.0029645
CTCTAGT9750.036.2586366
TCTAGTT10400.033.991617
CTAGTTG10650.032.86138
TACTCTA11150.032.6481554
GTACTCT11000.032.140373
GGGGAAA128450.030.35877268
GGGGGAA131400.029.97024367
GGGAAAA132600.029.91059569
GGAAAAA141900.028.93704870
TGTTACC12050.027.58668713
TGTACTC14050.025.414992
GTTACCT12900.024.68394914
TAGTTGT16100.023.0416879
TACCTCT13850.022.73846816
TTACCTC13900.022.6563915
GGGGGGA180550.022.00551466
ACCTCTA14700.021.42365617
TGGACAC15700.019.61606428
TTGTACT18450.019.1663991
Produced by FastQC (version 0.11.5)