FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T0_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T0_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103057
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101720.24791271483676683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG5900.027.2930075
CTCTAGT7750.022.1324446
GGGGGAA36350.021.38115767
TACTCTA7750.021.2295954
CACTAGT6350.020.39187236
GGGGGGA40800.019.90721366
CCACTAG6400.019.68981435
TCTAGTT11050.018.3734257
GTTACTA7100.018.2426913
GGGGAAA43350.018.09012468
ACTACCC7250.017.8648226
ACTAGTA7550.017.15119737
GTACTCT9650.017.0496753
CTAGTAG7550.016.6876538
TACTACC8100.015.9905095
TACCCTA8400.015.831728
TACACTC8400.015.8290213
TACCTCT10200.015.78001416
TGTTACC10550.014.92484313
CTACCCT8950.014.8622647
Produced by FastQC (version 0.11.5)