FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477288
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG977412.8108399419317585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG54170.15578232231555167No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC49050.14105820397965313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38890.11184003165685442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT8350.049.02963621
CCAATAG10200.039.45884738
ACTAGAG10850.037.73248720
TAGAGTA11350.036.37907822
TAGATCT7950.036.1030145
TAGAGGT11850.035.732539
AGAGGTA12050.035.71719410
GGTGGTC8550.035.61689453
TCGGTGG7900.035.44486651
AACTAGA11500.035.2955119
AGAGTAC11750.035.1406423
CAACTAG12050.033.6845118
GGTCGCC9100.033.46374556
CGGTGGT8800.033.41177752
ATTAGAG13400.031.8677757
GTGGTCG9350.031.82073654
GAGTGTC10300.031.59584628
GTGTAGA10000.031.50071742
CAATAGA13250.030.64000539
GAGGTAA14300.030.09735711
Produced by FastQC (version 0.11.5)