FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860492
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91570.32011975562245937No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG76920.2689047898053901No Hit
CAATTATGAAAAAAGCTGTCATTGTAGAAAACAAAGGTTGTGCAACATGC32610.11400136759690291No Hit
GTGAACTACTGTGGAAGTTACTGACGTAAGATTACGCCGCGTGAATACCG31840.11130952297716618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG500.00720620328.00472368
GCTAGAT3850.024.5560341
AAGGGAG24350.022.99257713
CTAACGT6850.022.4914421
CCTAGGT5400.022.0465371
TACCCCC11850.021.85220111
TTACCCC12050.021.4895110
AGGTGGT7400.021.2858856
CTTACCC12550.020.9147429
ACCCCCA12650.020.46988712
GGAGGAT28400.019.96012916
GGTGGTG8100.019.8778087
TATCGAC9350.019.841535
GTGGTGA8250.019.5136648
CACTAGG4350.019.31123764
TGGGTAC4550.019.2306537
AGTATAC8400.019.16822431
CACACTA4600.019.0226262
AAGGTGG8100.019.0142215
GGGTAAT5400.018.80441
Produced by FastQC (version 0.11.5)