FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666807
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2574945.51756265043744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG219380.4700858638465229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG61960.13276743606495833No Hit
GAAAAACAAAAAAAGGGAGGATTCAATTATGAAAAAAGCTGTCATTGTAG57990.12426054902206156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA329700.035.33969570
ACTCTAG6450.032.0203135
GGGAAAA356350.031.41922669
GGGGAAA362650.030.3028568
GGGGGAA378150.029.01447567
TACTCTA7350.028.0991574
CTAGTTG7750.027.0930048
CTCTAGT7200.026.7401266
TCTAGTT7900.025.2556047
GTACTCT8700.023.7389433
GGGGGGA522850.021.3997966
GAAAAAA243850.020.35878870
GGGGTAA30150.019.39139267
ACCTCTA10750.017.576517
GGGTAAA34700.017.3531768
TAGTTGT13400.017.2364089
TCGGTGG4700.017.12821451
TACCTCT11000.016.85894216
GGGGGTA33950.016.80844966
TGTACTC13000.016.4267752
Produced by FastQC (version 0.11.5)