FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_79T-1_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_79T-1_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4096000
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG642801.5693359375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG73410.1792236328125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG52140.12729492187500002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG50840.12412109375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG49120.119921875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG4650.040.6488235
GGAAAAA219200.032.0232870
GGGGAAA217250.029.87588168
GGGAAAA223750.029.83868269
GGGGGAA222550.029.11719967
ATAGGGT5400.027.86913737
CTCTAGT7150.027.4147196
GGATAGG5500.027.36576735
TCGGTGG5600.026.25108151
TACTCTA7250.026.0713124
GATAGGG6550.023.50983436
GGGGGGA282100.023.33058266
GTACTCT9050.023.206793
TCTAGTT10200.022.647997
GTTACTA9050.021.6596723
ACTACCC8800.021.0811866
TACTACC9500.020.2649845
TACCCTA9200.020.1599358
GGGGTAA21100.019.57808967
GGTCGCC8350.019.2822356
Produced by FastQC (version 0.11.5)