FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n02_659T-1_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n02_659T-1_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301610.6658837934424543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG5400.028.5195245
GGGGGAA71150.025.43645167
TACTCTA7050.023.8306274
GGGGAAA74050.023.73119268
GGGGGGA80650.023.52533366
ACTACCC7950.022.0128676
CTCTAGT7550.021.3247816
GGGAAAA85350.020.91739369
GTTACTA7650.020.5887323
TACTACC8000.020.5632215
TTACTAC9100.018.0775594
TCTAGTT10500.018.0002357
CTACCCT9400.017.8725747
TACCCTA9300.017.6868428
GGAAAAA107650.017.43015370
CACTAGT8550.017.19225936
GTACTCT9600.017.135833
TACACTC9550.016.85550913
CCACTAG8100.016.85225935
ACTAGTA9150.016.06525237
Produced by FastQC (version 0.11.5)