FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7509304
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370760.493734172967295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324890.4326499499820489No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT236580.3150491710017333Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT202250.2693325506598215TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT126410.16833783796740684TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT111380.14832266745360156TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT93710.12479185820683247TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT82680.11010341304600267TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC307850.057.8513521
CACACGT309950.057.75150712
GCACACG310850.057.55059411
TGAACTC311950.057.53664420
ACACGTC313700.057.21965413
CTCCAGT293500.057.09235824
CTGAACT316550.056.90604819
ACGTCTG312600.056.7821315
CACGTCT313950.056.6739114
AGCACAC322750.056.60170710
AACTCCA323600.056.33608222
CGTCTGA315750.056.29346516
AGAGCAC322650.056.0228168
CCAGTCA273050.055.96436726
ACTCCAG314800.055.32746523
CAGTCAC264200.055.30320727
TCCCTAC40350.055.2281539
CTCGTAT187450.054.9283444
TCTGAAC330950.054.69241318
AGTCACC84150.054.6810128
Produced by FastQC (version 0.11.5)