FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_sigF3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sigF3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766563
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC163530.34307739140340743No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC158540.33260863225766657No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG151510.3178600597537471No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA145330.30489474281573536No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG132600.2781878682816109No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT110170.23113090081889193TruSeq Adapter, Index 6 (97% over 36bp)
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA102990.21606763615628286No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT95420.20018617188108076No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC94700.19867564951937064No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG91880.19275943693600608No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG85440.17924865358959904TruSeq Adapter, Index 6 (97% over 36bp)
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC81800.17161212387206462No Hit
CTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTAGATG69040.14484231090620223No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT62170.1304294100382183No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT60770.127492283223782No Hit
AAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCG60510.1269468168153867No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA59860.12558315079439838No Hit
CCGCCAGCTGCTACAGCGCGGCGCAATTCAAAAAGGCGAGGTTTCTTATA58410.12254112659373222No Hit
GGGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCG57990.12165998854940133No Hit
TTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACCT55450.11633120132892401No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT52980.11114927044916852No Hit
GGAAAAGGATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGC49480.10380645341307772No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT48260.10124695718906893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG25500.054.76428243
ACTCCAG36100.052.3547223
ACTCCGG37450.049.8153232
CACACGT38400.049.39807512
ATCGGGG33800.049.39311242
GCACACG38400.049.2163111
GTCACTC38250.048.7688229
CACTCCG39050.047.8638631
AGTCACT39100.047.7086328
CCAGTCA40100.046.9577426
GAACTCC40650.046.66679821
CTCCGGA40150.046.63969833
TCACTCC40050.046.58136430
CGGGGGT17950.046.0149144
TGAACTC41450.045.85055520
AACTCCA41350.045.70750822
TCCAGTC41300.045.5933525
CAGTCAC41750.044.84807627
CTCCAGT42200.044.78685824
CTGAACT42900.044.298519
Produced by FastQC (version 0.11.5)