FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_sigE4.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sigE4.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571536
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT122402.1415973796926178TruSeq Adapter, Index 7 (97% over 35bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG6190.10830463872791914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG15500.063.45105728
TATCTCG14250.062.3908841
ATTCGTA15500.062.32748836
GTATGCC14400.062.2216847
ACTCCAG16000.061.6869623
CAGTCAC16150.060.897327
GCACACG16250.060.7379311
ACACGTC16400.060.39581313
CACACGT16350.060.3664412
GAACTCC16400.060.182421
TGAACTC16400.060.182420
TTCGTAT16150.060.03568337
TCCAGTC16400.059.96898725
TCGTATC16350.059.51538538
CTCCAGT16600.059.45731424
CGTCTGA16650.059.2787616
GAATTCG16400.058.90192434
CGAATTC16600.058.82478733
AACTCCA16750.058.71590422
CTGAACT16950.058.22957619
Produced by FastQC (version 0.11.5)