FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_sigE3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_sigE3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6041834
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2316073.8333890007570552TruSeq Adapter, Index 7 (97% over 36bp)
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG194970.32270002783922896No Hit
ATTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAAC146310.2421615688216525No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA138970.2300129397795438No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG128570.2127996234256022No Hit
GCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCA98410.16288100599917177No Hit
AAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGC92860.15369505352182797No Hit
TTTTTTTACAAGGAAGGAGGGAAAAGGATGAAAAAGGCTTATAAGAAACC92140.15250336238963202No Hit
GGCGTGATAGCCTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCC88900.14714075229475024No Hit
CTCATATAGCATTTGACATCTAGTACTCTATTTTAGGAGGGATGCTCAAT76220.1261537473555215No Hit
CTCAAACGTTAATCGTTTTGGATAAGATTAATGACCGCCAGCTGCTACAG73530.12170145687551163No Hit
GCTCAATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGT70550.11676917968947839No Hit
CGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGT62920.1041405639413463No Hit
GAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCATT61200.10129374623665595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG264400.064.7079223
GTATGCC264000.064.69614447
CACACGT268400.064.21969612
GCACACG268800.064.150711
GGCTATG266800.064.0146535
AGTCACC267200.063.91922428
CCAGTCA267450.063.83911526
CTATGAT265050.063.61899637
TCCAGTC268750.063.5693825
CCGGCTA269100.063.49813533
CTCGTAT267000.063.45693644
CGTATGC270500.063.26986346
TGAACTC271450.063.2594420
GCTATGA268550.063.2455636
ACACGTC273000.063.12478613
AACTCCA271800.063.06208422
TCGTATG271350.063.0329845
CTCCAGT271950.062.9114624
GAACTCC272850.062.85788721
CAGTCAC272250.062.8364227
Produced by FastQC (version 0.11.5)