FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_s8774.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_s8774.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497328
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT182610.7312215295708053TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG24300.053.01865841
ACTCCAG25150.051.91135823
AGTCACC25450.050.74422528
AGCTATC25600.050.4620538
GTCACCT25650.050.3475529
GAACTCC26150.049.7933621
CACACGT26650.049.11495612
GAAGCTA26800.048.72605536
CTCCAGT26650.048.59549724
GCACACG26950.048.56822211
GTATGCC27200.047.8654447
ACACGTC27400.047.77056513
CAGTCAC27200.047.7367727
TGAACTC27650.047.3452820
AACTCCA27800.047.3416422
CTATCTC28100.046.3463140
TCGTATG28350.045.9238145
AAGCTAT28300.045.8950637
TCCAGTC28250.045.8431925
AGCACAC28600.045.7662110
Produced by FastQC (version 0.11.5)