FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_csfG3.5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_csfG3.5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944719
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1213892.04196363192272TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC145700.060.72464828
ACTCCAG146350.060.67323323
GTATGCC147000.059.71346747
TATCTCG147000.059.62876541
CACACGT149900.059.53887612
CTCATTA149100.058.9772436
TGAACTC151950.058.7366120
GAACTCC151700.058.60268421
CTCCAGT151800.058.5179724
CGTATGC150050.058.45255746
CCAGTCA151750.058.42192526
ACACGTC153300.058.30970813
AACTCCA152450.058.26846722
TCGTATG150650.058.2662245
CTCGTAT151200.057.9848344
TCCAGTC153500.057.8698925
CAGTCAC153550.057.64298627
GCACACG154750.057.60357311
ATTATCT152600.057.37173539
ACGTCTG155950.057.31886715
Produced by FastQC (version 0.11.5)