FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_WT0_sigW.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_WT0_sigW.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5121067
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3626707.081922575900686TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT129290.25246691753886447TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG91540.17875181090190775TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT59160.11552280022893667TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG412700.065.460741
TCTCGTA404800.065.3532943
AGTCACG462700.064.79085528
GAACTCC463350.064.7705121
GTATGCC413150.064.7361947
CTCGTAT408150.064.67969544
TCGTATG403200.064.5449845
CGTATGC408900.064.4069946
GTATCTC421750.064.3376240
ATCTCGT416000.064.26681542
CGTATCT433250.064.2620839
CACGTCT468200.064.22597514
ACTCCAG467300.064.2155223
TGAACTC467600.064.21175420
CACACGT469150.064.1698912
ATTCGTA463500.064.1107436
GTCACGA467600.064.0969429
CCAGTCA467700.064.0913726
ACACGTC470000.064.0544713
GCACACG470550.063.95666511
Produced by FastQC (version 0.11.5)