FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3472199
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT36020.10373829380170894TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG15250.049.80201732
CTCGCGC16000.047.47712335
TCTCGCG16100.046.96006834
CTCTCGC16900.045.1467933
CACACGT17500.044.19888312
TCCAGTC18400.042.79906525
CTCCAGT18250.042.57549324
CGCGCAT17450.042.52898437
GCACACG18500.042.00014511
TCGCGCA18100.041.96872336
ACTCCAG18850.041.4059823
GTCACTC18350.041.38829
CCAGTCA19150.040.75732426
CAGTCAC19650.039.8972127
AGTCACT19600.039.8204228
GAACTCC19850.039.14427621
CACGTCT20000.038.67402314
ACACGTC20600.037.7174913
TCACTCT21550.035.56754330
GCGCATC22550.033.06569738
Produced by FastQC (version 0.11.5)