FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5166092
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT383360.742069634067686TruSeq Adapter, Index 7 (97% over 36bp)
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA68680.13294381904155017No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG66200.12814328509829093No Hit
GAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAG57230.11078006353738958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG52000.054.048623
GTATGCC52200.053.84099247
AGTCACC53050.052.84330428
CCGGCTA52850.052.51706333
CACACGT54000.052.43213712
TCACCGG53700.052.01167330
GCACACG54550.051.96714811
TCCAGTC54700.051.50872825
CTCCAGT54600.051.3466524
CCAGTCA54850.051.17643426
TCGTATG56100.050.4713945
GGCTATG56850.049.138835
ACACGTC57650.049.112513
CACGTCT57650.049.112514
CAGTCAC57300.049.10709827
CGTATGC58200.048.2894446
GCTATGA57850.048.28938336
CTATGAT58750.047.37041537
AGCACAC60750.047.1819910
GAACTCC59650.047.1765621
Produced by FastQC (version 0.11.5)