FastQCFastQC Report
Sat 2 Jun 2018
H3WGNAFXY_n01_VGT2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_VGT2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432278
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT274230.6187111909496652TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT96710.2181947973480003TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGTAT50710.11441069355306684TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGTAT49470.11161303510294254TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGAT46070.1039420361267953TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC94400.061.5221535
ACTCTCG95200.061.25150332
CTCTCGC95250.061.18260233
GTCACTC96450.060.8150829
TCACTCT96550.060.72200430
ACTCCAG97250.060.53823523
TCTCGCG97350.059.83021534
AGTCACT98550.059.6257128
CGCGCAT97350.059.01122337
CCAGTCA99800.058.92060526
CAGTCAC100450.058.56691727
CTCCAGT101000.058.2905324
CACTCTC100650.057.96962431
GAACTCC102850.057.37881521
TCGCGCA101000.057.32858736
CACACGT103400.057.27025212
TCCAGTC103700.056.9409625
GTATGCC47100.056.77293447
GCACACG104400.056.62047611
TGAACTC104250.056.50753420
Produced by FastQC (version 0.11.5)